Biosynthesis of polycyclopropanated high energy biofuels

Cruz-Morales, P.; Yin, K.; Landera, A.; Cort, J.; Young, R.; Kyle, J.; Bertrand, R.; Iavarone, A.; Acharya, S.; Cowan, A.; Chen, Y.; Gin, J.; Scown, C.; Petzold, C.; Araujo-Barcelos, C.; Sundstrom, E.; George, A.; Liu, Y.; Klass, S.; Nava, A.; Keasling, J. (2022). Biosynthesis of polycyclopropanated high energy biofuels. Joule, 6(7), 17.https://doi.org/10.1016/j.joule.2022.05.011

@article{Cruz-Morales-2022-3963,
author = {Cruz-Morales, P and Yin, K and Landera, A and Cort, J and Young, R and Kyle, J and Bertrand, R and Iavarone, A and Acharya, S and Cowan, A and Chen, Y and Gin, J and Scown, C and Petzold, C and Araujo-Barcelos, C and Sundstrom, E and George, A and Liu, Y and Klass, S and Nava, A and Keasling, J},
title = {Biosynthesis of polycyclopropanated high energy biofuels},
journal = {Joule},
year = {2022},
month = {jul},
publisher = {Cell Press},
volume = {6},
number = {7},
pages = {17},
doi = {10.1016/j.joule.2022.05.011},
url = {https://www.sciencedirect.com/science/article/pii/S2542435122002380},
keywords = {Unspecified Feedstock, Biodiesel (FAME), Unspecified Pathway, Fuel Properties and Characteristics},
}

TY - JOUR
TI - Biosynthesis of polycyclopropanated high energy biofuels
AU - Cruz-Morales, P
AU - Yin, K
AU - Landera, A
AU - Cort, J
AU - Young, R
AU - Kyle, J
AU - Bertrand, R
AU - Iavarone, A
AU - Acharya, S
AU - Cowan, A
AU - Chen, Y
AU - Gin, J
AU - Scown, C
AU - Petzold, C
AU - Araujo-Barcelos, C
AU - Sundstrom, E
AU - George, A
AU - Liu, Y
AU - Klass, S
AU - Nava, A
AU - Keasling, J
T2 - Joule
AB - Cyclopropane-functionalized hydrocarbons are excellent fuels due their high energy density. However, the organic synthesis of these molecules is challenging. In this work, we produced polycyclopropanated fatty acids in bacteria. These molecules can be converted into renewable fuels for energy-demanding applications such as shipping, long-haul transport, aviation, and rocketry. We explored the chemical diversity encoded in thousands of bacterial genomes to identify and repurpose naturally occurring cyclopropanated molecules. We identified a set of candidate iterative polyketide synthases (iPKSs) predicted to produce polycyclopropanated fatty acids (POP-FAs), expressed them in Streptomyces coelicolor, and produced POP-FAs. We determined the structure of the molecules and increased their production 22-fold. Finally, we produced polycyclopropanated fatty acid methyl esters (POP-FAMEs). Our POP fuel candidates can have net heating values of more than 50 MJ/L. Our research shows that the POP-FAMEs and other POPs have the energetic properties for energy-demanding applications for which sustainable alternatives are scarce.
DA - 2022/07//
PY - 2022
PB - Cell Press
VL - 6
IS - 7
SP - 17
UR - https://www.sciencedirect.com/science/article/pii/S2542435122002380
DO - 10.1016/j.joule.2022.05.011
LA - English
KW - Unspecified Feedstock
KW - Biodiesel (FAME)
KW - Unspecified Pathway
KW - Fuel Properties and Characteristics
ER -